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Table 3 The primers used to construct the tagged strains T-prgI, T-sipA, T-sipB, T-sopE2, T-spaO, and T-sptP.

From: Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice

ORFs

Upstream primer

Downstream primer

prgI

5'ATAACTTGTACCGTAACGCGCAA TCGAACACGGTAAAAGTCTTTAAG GATATTGATGCTGCCATTATTCAGA ACTTCCGTGATTACAAGGATGACG ACGA 3'

5'GACCTGATATTGACCGCCTGCCC TATAACGGCATTCTCAGGGACAAT AGTTGCAATCGACATAATCCACCTT ATAACTGACATATGAATATCCTCCT TAGTT 3'

sipA

5'GCCCGGCTTACGAGTCATACCTG AATAAACCTGGCGTGGATCGGGTT ATTACTACCGTTGATGGCTTGCACA TGCAGCGTGATTACAAGGATGACG ACGA 3'

5'ACATCAACGGCAATACAAGAGG TGATCACTTTTTTGACTCTTGCTTCA ATATCCATATTCATCGCATCTTTCC CGGTTAACATATGAATATCCTCCTT AGTT 3'

sipB

5'CGGAACTGCAAAAAGCCATGTCT TCTGCGGTACAGCAAAATGCGGAT GCTTCGCGTTTTATTCTGCGCCAGA GTCGCGCAGATTACAAGGATGACG ACGA 3'

5'GAATGATTATTTAAATAAGCGGC GGGATTTATTCCCACATTACTAATT AACATATTTTTCTCCCTTTATTTTGG CAGTTTCATATGAATATCCTCCTTA GTT 3'

sopE2

5'ATAAGGGGACGATGCCAACGCC ACAACAATTTCAGTTAACTATAGA AAATATTGCGAATAAGTATCTTCAG AATGCCTCCGATTACAAGGATGAC GACGA 3'

5'TCGCCATAAAAACGAATATATAG TTTCAGAAAATCTGCTATTAATTCA TATGGTTAATAGCAGTATTGTATTT ACTACCACATATGAATATCCTCCTT AGTT 3'

spaO

5'ATGAATGACACCTTAGGCGTTGA GATCCATGAATGGCTGAGCGAGTC TGGTAATGGGGAAGATTACAAGGA TGACGACGA 3'

5'CCTGACGCAATAATAAATGGCAA CAGGGTGGAAAATGCCAGTAAGGC AATTAATGAGATACATATGAATAT CCTCCTTAGTT 3'

sptP

5'GCCTCACAGTTTGTACAACTAAA AGCAATGCAAGCCCAGTTGCTTAT GACGACGGCAAGCGATTACAAGGA TGACGACGA 3'

5'CAAATAATTATACAGAAATAGCT TACTTTCAGATAGTTCTAAAAGTAAG CTATGTTTTTACATATGAATATCCTC CTTAGTT 3'