Table 1 The bacterial strains and plasmid constructs used in the study
Bacterial strains, plasmids | Â | Description | Reference/source |
|---|---|---|---|
S. typhymurium strains | ST14028s | Wild type and parental strain | Â |
| Â | T-prgJ | prgJ::1xFLAG | This study |
| Â | T-sipA | sipA::1xFLAG | This study |
| Â | T-sipB | sipB::1xFLAG | This study |
| Â | T-sopE2 | sopE2::1xFLAG | This study |
| Â | T-spaO | spaO::1xFLAG | This study |
| Â | T-sptP | sptP::1xFLAG | This study |
E. coli s train | DH5a | F-Φ80dlac ZΔM15Δ(lac ZYA-argF)U169deoRrecA1endA1hsdR17(rk-mk+)phoAsupE44λ-thi-1gyrA96relA1 | Invitrogen |
Plasmids | pUC-H1PF1 | Apr and Kanr, template plasmid for 1xFLAG epitope tag | [43] |
| Â | Kan-clone7 plasmid | Derived from pkD4, containing a kanamycin resistance cassette and sequence which can be recognized by flapase | [44] |
|  | pkD46 | Apr, containing the Red recombinase of λ phage | [44] |
| Â | pCP20 | Containing the expression cassette of flapase which can remove the kanamycin resistance cassette from the mutant strains | [44] |