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Figure 6 | BMC Microbiology

Figure 6

From: Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice

Figure 6

Western analyses of the expression of the tagged proteins from the internalized bacterial strains T-prgI (lane 1), T-sipA (lane 2), T-sptP (lane 3), T-spaO (lane 4), T-sopE2 (lane 5), and T-sipB (lane 6) recovered from spleens. BALB/c mice were intraperitoneally infected with 1 × 105 CFU of the tagged strains, and internalized bacteria were recovered from the spleens at 5 days post inoculation. The expression of bacterial DnaK was used as the internal control (B). Protein samples were reacted with antibodies against the FLAG sequence (A) and DnaK (B). Each lane was loaded with material from 5 × 107 CFU bacteria.

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BMC Microbiology

ISSN: 1471-2180

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