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Figure 1 | BMC Microbiology

Figure 1

From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair

Figure 1

N. meningitidis (Mc) Fpg. (A) Physical map of the Mc fpg open reading frame and flanking regions. The fpg gene contains a DNA uptake sequence (DUS). Primers KT1b and KT2b employed in cloning of the Mc fpg gene are depicted. The gene organization of the Mc fpg flanking regions is identical in all available neisserial genomes. NMB1296 encodes a hypothetical protein with sequence homology to DNA methyltransferases. A promoter is predicted upstream of NMB1296 (black arrow). The fpg and the lysophophatidic acid acyltransferase nlaA genes are putatively co-transcribed [27], although an inverted repeat (containing DUS) associated with transcription termination or attenutation is found downstream of the fpg gene. NMB1297 is COG-annotated mltD (membrane-bound lytic murein transglycosylase). NMB1293 is a hypothetical protein. The distribution of DUS and degenerate DUS is indicated. (B) Structural modeling of Mc Fpg based on E. coli Fpg (PDB 1k82) showing the DNA binding motifs helix-two-turn-helix (H2tH) (blue) and zinc finger (orange), as well as the N-terminal domain (green) containing the glycosylase catalytic amino acid residues. Amino acids encoded by DUS are highlighted in purple.

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