Skip to main content

Table 2 DNA fragmentation levels obtained in strains of E. coli with different susceptibilities to CIP.

From: Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay

    CIP dose
Strain Mutations MIC MIC 1× MIC 10× MIC 100×
C-20 - 0.007 1.5 ± 0.3 6.7 ± 0.8 10.3 ± 2.5
C-15 Ser83Leu from GyrA 0.25 1.7 ± 0.3 6.2 ± 0.7 8.7 ± 1.1
1273 Ser83Leu and Asp87Tyr from GyrA 8 0 1.8 ± 0.3 2.7 ± 0.4
1383 Ser83Leu and Asp87Tyr from GyrA and Ser80Ile and Glu84Lys from ParC 128 0 0 0
J53 - 0.007 1.8 ± 0.8 9.2 ± 1.2 10.4 ± 2.0
J53qnrA1 Plasmid gene J53qnrA1 0.25 1.9 ± 0.4 9.5 ± 1.3 9.8 ± 0.9
  1. The level of fragmentation obtained by different CIP doses is indicated by the width of the halo of dispersion of DNA fragments and is measured in μm (mean ± standard deviation). MIC is in μg/ml.