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Table 1 Dose-response effect of CIP on TG1 E. coli chromosomal DNA analyzed with the Micro-Halomax® kit.

From: Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay

Dose (μg/ml)

Width of dispersion (μm)

Class

Range

0

-

  

0.003

-

0

0

0.006

-

  

0.008

-

  

0.012

1.3 ± 0.3

I

≤ 2.0

0.02

1.6 ± 0.3

  

0.04

2.5 ± 0.4

II

2.1 – 3.7

0.08

3.3 ± 0.4

  

0.1

5.1 ± 1.0

III

3.8 – 5.7

0.5

7.8 ± 1.4

IV

≥ 5.8

1

8.8 ± 1.6

  
  1. The width of the halo of dispersion of DNA fragments is presented in μm (mean ± standard deviation). The extent of DNA damage was classified according to the width of the dispersion.