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Table 1 Dose-response effect of CIP on TG1 E. coli chromosomal DNA analyzed with the Micro-Halomax® kit.

From: Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay

Dose (μg/ml) Width of dispersion (μm) Class Range
0 -   
0.003 - 0 0
0.006 -   
0.008 -   
0.012 1.3 ± 0.3 I ≤ 2.0
0.02 1.6 ± 0.3   
0.04 2.5 ± 0.4 II 2.1 – 3.7
0.08 3.3 ± 0.4   
0.1 5.1 ± 1.0 III 3.8 – 5.7
0.5 7.8 ± 1.4 IV ≥ 5.8
1 8.8 ± 1.6   
  1. The width of the halo of dispersion of DNA fragments is presented in μm (mean ± standard deviation). The extent of DNA damage was classified according to the width of the dispersion.