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Table 2 Phylogenetic affiliation of OTUs and sequences of the %G+C fractioned libraries and the unfractioned library.

From: Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria

Library

Fractioned G+C 25–75%

Unfractioned

Group

OTUs

n (%)

Sequences

n (%)

OTUs

n (%)

Sequences

n (%)

Phylum Firmicutes

323 (71.0)

2190 (68.5)

113 (86.3)

428 (93.2)

Clostridium cluster IV

107 (23.5)

753 (23.5)

36 (27.5)

131 (28.5)

Clostridium cluster XIV

131 (28.8)

1057 (33.0)

52 (39.7)

233 (51.0)

Enterococcaceae

2 (0.4)

5 (0.2)

0 (0)

0 (0)

Lactobacillaceae

4 (0.9)

34 (1.1)

0 (0)

0 (0)

Staphylococcaceae

2 (0.4)

2 (0.1)

0 (0)

0 (0)

Streptococcaceae

6 (1.3)

20 (0.6)

2 (1.5)

5 (1.1)

Other Firmicutes

71 (15.6)

311 (9.7)

22 (16.8)

58 (12.6)

Phylum Actinobacteria

65 (14.3)

851 (26.6)

8 (6.1)

16 (3.5)

Actinomycetales

10 (2.2)

24 (0.8)

0 (0)

0 (0)

Bifidobacteriales

17 (3.7)

398 (12.4)

5 (3.8)

11 (2.4)

Coriobacteriales

38 (8.4)

429 (13.4)

3 (2.3)

5 (1.1)

Phylum Bacteroidetes

37 (8.1)

99 (3.1)

8 (6.1)

13 (2.8)

Phylum Proteobacteria

24 (5.3)

42 (1.3)

1 (0.8)

1 (0.2)

Alphaproteobacteria

3 (0.7)

6 (0.2)

0 (0)

0 (0)

Betaproteobacteria

9 (2.0)

16 (0.5)

0 (0)

0 (0)

Deltaproteobacteria

5 (1.1)

11 (0.3)

0 (0)

0 (0)

Gammaproteobacteria

7 (1.5)

9 (0.3)

1 (0.8)

1 (0.2)

Other phylaa

6 (1.3)

17 (0.5)

1 (0.8)

1 (0.2)

Sum

455

3199

131

459

  1. a. Affiliation with Acidobacteria, Cyanobacteria, TM7 and Verrucomicrobia