BMC Microbiology

Table 1 Characteristics of the sequence libraries.

From: Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria

Library(s)	Sequences (no.)	OTUs (no.)^a	%G+C^b	Singletons (no.)	Coverage^c
Fr G+C 25–30%	319	91	51.5	43	87
Fr G+C 30–35%	350	94	52.6	48	86
Fr G+C 35–40%	313	93	53.4	50	84
Fr G+C 40–45%	346	119	53.9	67	81
Fr G+C 45–50%	316	112	56.0	62	80
Fr G+C 50–55%	292	62	58.1	22	93
Fr G+C 55–60%	311	45	62.1	22	93
Fr G+C 60–65%	303	64	61.7	26	91
Fr G+C 65–70%	362	130	57.6	65	82
Fr G+C 70–75%	287	116	55.5	67	77
Fr G+C 25–75%^d	3199	455	56.2	180	94
Unfractioned	459	131	53.6	66	86

a. The number of OTUs determined with DOTUR using 98% similarity criterion [53]
b. Average %G+C content of the partial 16S rRNA gene sequences
c. Coverage according to Good [23]
d. The combined G+C fractions

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ISSN: 1471-2180

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