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Table 2 Oligonucleotide primers used in this study

From: Transcription profiles of hydrogenases related genes in the cyanobacterium Lyngbya majuscula CCAP 1446/4

Primera

Sequence 5' → 3'

Primera

Sequence 5' → 3'

GWhox1R

ATCAGCACCTCGTCCAGCAACATCCC

LmhupW1R

CGCAGTTCCGCAGTCAAAGATTCGCA

GWhox2F

CGCTAAGTTACCCGAAGAAGCCGCCAC

LmhupW2R

TCAAAAACTGCACCGGGTTCT

GWhox3R

CAACTTCTCGCTTAGGAAATATGTAGGG

LmhupW3R

GGTTCGGGTAATTGTTCAAGCTCT

GWhox4R

CTAAATGACAGAGGACGGGAATATGAACC

LmHCPR

GGTTGATAACAACTGATCTCAGACCAT

GWhox5R

ACCTTCTTCTACGGCAACGTCAATGTCG

LmhoxHF d

CGTGCGATCGCCCTTATCAGAAGA

GWhox6F

GAGACCTCTATCACGATACTGTCCGCAC

LmhoxHR d

AGTTAGAATTAATTGCGGAAAACCTC

GWhox7R

TAAGCCAATATCTTGACCCTGTTGTGC

LmhoxUF1

CGACATTGACGTTGCCGTAGAAGAAGG

GWhox8R

GATGGGGATCACTTTCAAGGACACTGCG

LmhoxYF

GATGTCACGCCGCAAATTCCGAAA

GWhox9R

GAAGGGACTTTCACGATAACGAGCAG

LmhoxWorfF1

TTATTCAAACTTTACGCAAGTCAAGGT

GWhox10F

AGGAAAGGTAGTAGTGAAACAACCTG

LmhoxWR2

CTGATGGAGTGCTAAACATTTCACAT

GWhoxW1F

CTCCATCAGTTGCTTCCTGAAGTTG

LmxisHR1

CAAATCTACAAACGTGGAACGTCC

GWhoxW1R

GATGTCCCCAGTTAAGTTCAGTTGTTTC

LmxisHR2

GCAAGATAAAGATAACGGTCTGGT

GW5Lmhox2R

TTTGCGGCGTGACATCACCTAACTCTA

LmxisHR3

CGTCTGTAATTGTCCAACCCTCTT

GW3LmhupWR1

TTTAGATTCCCACAACCAATAATCG

LmxisHR4

AATCTCGTTCAGCTGCAATGAGTT

LahoxWF1

TTCGGGGTGATGATGG

RChoxE1R

ACCAGGGAAACTAAACCGTC

LahoxWR1

GGAACTGATATTAACCAAGC

RChoxE2R

TGAGGGAAAACAAATGGTAAAAGG

BD16SF1

CACACTGGGACTGAGACAC

RChoxE3R

GGAAGTTTGAGGTTACGGGCGAC

BD16SR1

CTGCTGGCACGGAGTTAG

RChoxE4R

GGTAAGGCACGGTTATTCC

BDhoxHF1

GATGATGCGGGCGAAGTTG

RThoxE1F

TTGGGGCTTGTGGGATTGC

BDhoxHR1

AGCGAGTAGGTGACTAACGG

RThoxE2F

TGAAACGCAGCCAATATCGT

BDhupLF1

ACACAAGCCCAACTATTTC

RThoxF1F

AGTCAAAGCCGTACAAACAG

BDhupLR1

CCAAGCGGTATCTAATGC

RThoxF2F

CAGTCGTCAACTGTTAGTCGTTCG

VNhoxWF1

CTCAAAAAACTGTCTTAGTGTTGG

RThoxY1F

ACGATTTGGTTAGCGGGTTGT

VNhoxWR1

GCTAAACATTTCACATTGGGAA

Sqhox5R1R

ATCACAACCGCCTACCAAGA

BDhupWF1

CTTTGGCTGCGGGTCGTC

Sqhox6F1R

CAACACCCATCACGGTCT

BDhupWR1

GATAATGCTGCTGTTGAGGTGATG

Sqhox8R1R

TCTATGTCAACCCGCCCA

LMS3'A b

TCCCTTCCATGACCTCAAAC

Sq8.2

TGCGCCTGTGGTTGTCTACGATG

LMH2B b

GCCGCAAATTCCACAAACTCG

Sq9RR1

GGAACTCAACACAACACAG

LMH4'A c

GGCTATCTCCTTAACGAAGA

Sq10F1F

CGACAAGTTGAAGCAAGGAAG

LMH4'BF b

ATCTGCGTCAGTTAGCCCGA

SqWF1

CTGTATTTGTAAGAGTTGCC

LMH5A b

GTGCAACAGAAGCCAGTCGC

SqWF2

CGGCTTCATGGTTAAAGTC

  

SqWF3

AGATCAGGAGGCGGATAAAC

  1. aF or A (forward) and R or B (reverse) designations refer to primer orientations in relation to the frame of the gene.
  2. bLeitão et al. [2].
  3. cSchütz et al. [4].
  4. dFerreira et al. [1].
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BMC Microbiology

ISSN: 1471-2180

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