hup genes physical map, hupW promoter, and analysis of cotranscription in Lyngbya majuscula CCAP 1446/4. (A) Physical map of L. majuscula hup genes (adapted from , accession number GenBank:AF368526), (B) analysis of the hup genes cotranscription by RT-PCR, and (C) nucleotide sequence of the promoter region upstream of hupW. A schematic representation of the cDNA and the products generated in the RT-PCRs is depicted below the physical map. Lanes 1: RT-PCR; Lanes 2: Negative control without reverse transcriptase; Lanes 3: Negative control (no template); Lanes 4: PCR positive control (genomic DNA); M: GeneRuler DNA Ladder mix (Fermentas). Within the hupW promoter region the following regions are indicated: a putative IHF binding site (boxed with the mismatching nucleotide shaded), the -10 and -35 boxes and the ribosome binding site – RBS (underlined), the transcription start point (+1, bold and underlined), and the start codon of hupW (bold and underlined).