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Figure 4 | BMC Microbiology

Figure 4

From: Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein

Figure 4

Competitive Electromobility shift analysis. EMSAs were performed with unlabeled competitors added to the reactions. 480 pmol IsaB and 270 pmol labeled RNA were included in each sample. Lane 1, labeled probe alone, Lane 2, IsaB + labeled RNA, Lane 3, IsaB + labeled RNA and 270 pmol unlabeled DNA, Lane 4, IsaB + labeled RNA and 270 pmol dNTPs, Lane 5, IsaB + labeled RNA and 270 pmol yeast tRNA.

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