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Figure 2 | BMC Microbiology

Figure 2

From: Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein

Figure 2

Cellular localization of IsaB by Western blot analysis. Sa113 and Sa113ΔisaB::erm cultures were fractionated into: spent medium (lanes 1 and 2), cell wall associated (lanes 3 and 4), cell membrane (lanes 5 and 6) and cytoplasmic (lanes 7 and 8) fractions. IsaB bands were observed in both the spent medium and cell wall associated fractions in wild-type Sa113 (lanes 1 and 3, arrows) but not in Sa113ΔisaB::erm (lanes 2 and 4 respectively). Proteins that reacted non-specifically with IsaB antiserum were observed in all lanes, but were present in the isaB mutant as well as wildtype.

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BMC Microbiology

ISSN: 1471-2180

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