a, Induction of P24, P21 and P16 productions in G. thermoleovorans B23. Cells were cultivated in LBM supplemented with 0.1% alkane mixtures (V/V) for 14 days at 70°C. Total cell fractions were loaded on an SDS-12% polyacrylamide gel. Protein bands were stained with Coomassie Brilliant Blue R-250. Lanes are molecular size marker, M; cultures after 0 day, 0; 6 days, 6; 8 days, 8; 10 days, 10; 12 days, 12; 14 days, 14; and 14 days cultivation in the absence of alkanes, -. b, Relative degradation of alkanes by strain B23. Fractions degraded were estimated by the reduction of peak areas in GC/FID.