Table 3 Primers used in this study
From: Characterization of oxylipins and dioxygenase genes in the asexual fungus Aspergillus niger
Sequence 5' → 3' | |
|---|---|
Constructs of complete genes | |
pMW012 | |
ppoA-dw | GAGGTGGGTCTTGTTTG |
ppoA-up | GACAAACAGGGAGTTGC |
pMW036 | |
ppoD-dw | GATTTCTTCCAGCTGGC |
ppoD-up | GCTACAGCTACAGCTAC |
Disruption constructs | |
pMW051 | |
ppoA3'-Nsi I-dw | ATGCATGGTGGCAAACCAAGCC |
ppoA3'-Kpn I-up | GGTACCGGTGAGGAGCACTACTTG |
ppoA5'-Hind III-dw | AAGCTTATTTGTAGAGTCGAGG |
ppoA5'-Sph I-up | GCATGCCATGCTTACCGTGAATG |
pMW061 | |
ppoD5'-Kpn I-dw | GGTACCTTCCAGCTGGCATTGGTG |
ppoD5'-Bam HI-up | GGATCCGTGCAGGGCCTTGAGCC |
ppoD3'-Sph I-dw | GCATGCTGAAGCGCAACGTCTAAC |
ppoD3'-Hind III-up | AAGCTTCAGCCCGTAGTTCTG |