Strain | CW | CCW | CW (%) |
|---|
S9 | 290 | 210 | 58 |
S9Δ1 C1 | 494 | 6 | 99 |
S9Δ1 C2 | 481 | 19 | 96 |
S9Δ2 | 500 | 0 | 100 |
S9Δ4 | 511 | 498 | 51 |
S9Δ2–4 | 499 | 1 | 100 |
- The flagellar rotational direction was analyzed by dark-field microscopy. Cells with clockwise (CW) rotating flagella are pushed forward by their right-handed flagellar bundle, whereas cells with counterclockwise (CCW) rotating flagella are pulled backward [53]. The flagella and the direction of movement of the cell can be seen under the dark-field microscope and thus the rotational direction be determined. Shown is the number of cells in CW and CCW swimming mode at the time point of observation, as well as the percentage of CW swimming cells.
