Figure 2

Immunofluorescence double staining of ADAM8 and HPIV2 marker of HPIV2 stimulated HSY cell cultures on culture day 0 (panel B), 1 (panel C), 3 (panel D). ADAM8 staining is shown in red and HPIV2 shown in green (arrowheads), together with the blue nuclear counterstain of the same field. Panel A shows the staining of HSY cells that HPIV2 did not infect as negative control, therefore (A) only ADAM8 weak staining with nuclear counterstain, (B, C, D) overlay of double staining of ADAM8 and HPIV2 marker with blue nuclear counterstain of the same field on culture days 0, 1 and 3, respectively. Bar = 10 μm.