Figure 3

Illustrations of the hupW operons. The hupW operon and surrounding genes in Nostoc PCC 7120 and Nostoc punctiforme. A. The transcription start point (TSP) and promoter region of hupW in Nostoc PCC 7120 together with the result from the reverse transcription (RT) reaction and subsequent PCRs. The positions of primers used in the experiments are shown (Table 1). (+): PCR-fragment, (-): negative control without RT enzyme, gDNA: positive control with gDNA. B. Schematic presentation showing TSP and promoter region of hupW together with RT-PCR detection of hupW transcripts in Nostoc punctiforme. The positions of primers used are shown (Table 1). (+): PCR-fragment, (-): negative control without RT, gDNA: positive control with gDNA. Results of PCR were visualized on a 1% agarose gel.