Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator ( agr ) locus

BMC Microbiology

Table 2 Summary of nucleotide changes and predicted outcomes of mutations in the agr locus.

Strain number	Amount of TSST-1 produced (μg/ml)	Changes in agrC region nucleotide sequence	Predicted outcome	tstpromoter	*sar*A	*sig*B
1	3.5	NC		NC	NC	NC
2	14	T(321) insertion	Frameshift→Truncated AgrC	NC	NC	NC
3	5	T 281A	phe→ile	NC	NC	NC
7	2	NC		NC	NC	NC
8	4	Δ495~504	Deletion of leu-lys-ile	NC	NC	NC
9	2.8	NC		NC	NC	NC
10	14	G651T	glu→his	NC	NC	NC
11	12	NC		NC	NC	NC
16	1.3	NC		NC	NC	NC

Data are from DNA sequencing of agr loci, tst promoter region, sarA and sigB from 9 strains. All mutations were found in agrC. NC, no change.