Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator ( agr ) locus

Table 1 Production of TSST-1 evaluated by Western blotting.

No.	Strain	μg/ml	No.	Strain	μg/ml
1	N315	3.5 ± 0.22	18	2680	1.4 ± 0.19
2	A36	14 ± 1.01	19	2681	1.3 ± 0.05
3	3429	5 ± 0.12	20	2682	1.0 ± 0.25
4	3472	1.3 ± 0.31	21	2683	1.0 ± 0.01
5	3337	1.1 ± 0.20	22	2684	0.8 ± 0.02
6	1785	1.2 ± 0.02	23	2685	1.6 ± 0.23
7	2271	2.0 ± 0.03	24	2686	2.0 ± 0.18
8	3281	4.0 ± 0.22	25	2687	1.6 ± 0.22
9	2932	2.8 ± 0.19	26	2688	7.6 ± 0.07
10	3543	14 ± 1.21	27	2689	9.8 ± 0.28
11	3573	12 ± 0.20	28	2690	3.1 ± 0.16
12	V432	7.0 ± 0.25	29	2701	5.0 ± 1.12
13	V637	7.6 ± 0.30	30	2702	2.8 ± 0.23
14	V666	5.2 ± 0.11	31	2165	4.0 ± 0.13
15	V700	8.0 ± 0.21	32	3624	1.0 ± 0.19
16	V723	1.3 ± 0.34	33	3878	2.2 ± 0.20
17	V694	4.0 ± 0.22	34	3890	6.4 ± 0.08

Strain 1, genome strain. Strains 2 to 34 were randomly selected clinical MRSA isolates that were all tst-positive and assigned to agr class 2 by PCR. Amounts of TSST-1 varied among strains and ranged from 0.8 to 14 μg/ml.