Table 1 Primer sequences

IdeR F

IdeR R

5'TTGGATCC ATGAACGATCTTGTCGATAC-3'

5'-CGGAATTC TCAGACCTTCTCGACCTTG-3'

cloning of ideR coding region into pGEX-6P-1

RG329

RG330

5'-CCGGGATCC ATGACGGGCGCGGT-3'

5'-CCGGAATT CTCACGTCTGGTTCCCG-3'

cloning of zur coding region into pGEX-6P-1

Rv0282-1

Rv0282-2

5'-CGGGATCC CGCAACACCCTGGTC-3'

5'-CGGGTACC CGCTGTCTCCTTCACC-3'

EMSA on rv0282 promoter region

mmp3

mmp7

5'-GCACGCTTGAGAGTTCC-3'

5'-TGCCACTTTCGGGTC-3'

EMSA on mmpS5 promoter region

Pr1MS F

Pr1MS R

5'-CCAGTACT GACGCTGGAACGAGTG-3'

5'-CCAAGCTT CTGACCACATCGCGG-3'

EMSA and cloning of msmeg0615 promoter region into pMYT131

Pr2MS F

Pr2MS R

5'-CCAGTACT ACGCTGACCGGCGAC-3'

5'-CCAAGCTT CTCATGACTGTTTCCTTTC-3'

Cloning of msmeg0620 promoter region into pMYT131

Pr2MT F

Pr2MT R

5'-CCAGTACT CAACGAGCCCGAGGCG-3'

5'-CCAAGCTT CTCATAACATCTCTCC-3'

Cloning of rv0287(esxG) promoter region into pMYT131

RA1

RA2

5'-GACCACGCGTATCGATGTCGAC(T)₁₆V-3'

5'-GACCACGCGTATCGATGTCGAC-3'

5' RACE PCR reactions

Ms0615-RT

MS0615-1

Ms0615-2

5'-GTCGACGACGGCCGGGGTG-3'

5'-CCGATCCACGCGTCGCAC-3'

5'-GTCGTGTGCGAGATGGGTC-3'

5' RACE for msmeg0615

Ms0620-RT

Ms0620-1

Ms0620-2

5'-GTCGAGCAGCGCATTGAC-3'

5'-CGAGACCTCGACGAAACG-3'

5'-GCATGCGCGGCCTGGAAG-3'

5' RACE for msmeg0620

Ms0615 A

Ms0615 B

5'-GGCCTGACGGTCAACG-3'

5'-ATCCACGCGTCGCACT-3'

qPCR for msmeg0615

Ms0620 E

Ms0620 F

5'-CAGGCCGCGATGAGTT-3'

5'-TCGAGCAGCGCATTGA-3'

qPCR for msmeg0620

mysA F

mysA R

5'-CGTCGCCGATGGTCTG-3'

5'-CCACGCCCGAAGAGC-3'

qPCR for M. smegmatis sigA (mysA)

Rv0282 C

Rv0282 D

5'-AGGTGTCGCGGCTGAA-3'

5'-GGTCCCGCAAACACCA-3'

qPCR for Rv0282

Rv0287 A

Rv0287 B

5'-CTGATGGCGGCACACGA-3'

5'-CAGAAACCGGGCATGG-3'

qPCR for Rv0287

sigA F2

sigA R2

5'-CGCGAAAAACCATCTG-3'

5'-GATCAGCCCCAGGTTG-3'

qPCR for tuberculosis sigA