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Table 1 Strains and plasmids used in this study

From: RpoE fine tunes expression of a subset of SsrB-regulated virulence factors in Salmonella enterica serovar Typhimurium

Strain or plasmid Genotype or description a Reference
Plasmids   
pKD46 repA101ts, ori R101, araC-ParaB-gam-bet-exo, bla [28]
pCP20 FLP+, λ cl857+ λ pR Repts, AmpR, CmR [28]
pKD3 pANTSy derivative, FRT-flanked cat from pSC140, CmR [28]
prpoE rpoE with endogenous promoters in pWSK29, AmpR This work
psrfN-2HA srfN with endogenous promoter and C-term. tandem HA fusion in pWSK129, KanR Our collection
psseL-2HA sseL with endogenous promoter and C-term. tandem HA fusion in pWSK129, KanR [8]
psseL-2HA sifA with endogenous promoter and internal tandem HA fusion in pACYC184 [37]
pIVET5n tnpR-lacZ, sacB, R6K ori, bla, AmpR [29]
pPsseA-lacZ sseA promoter fused to tnpR-lacZ in pIVET5n, AmpR [29]
pPsseL-lacZ sseA promoter fused to tnpR-lacZ in pIVET5n, AmpR This work
pPsrfN-lacZ srfN promoter fused to tnpR-lacZ in pIVET5n, AmpR This work
pPsifA-lacZ sifA promoter fused to tnpR-lacZ in pIVET5n, AmpR This work
pPssaB-lacZ ssaB promoter fused to tnpR-lacZ in pIVET5n, AmpR This work
pPssaG-lacZ ssaG promoter fused to tnpR-lacZ in pIVET5n, AmpR This work
Strains   
SL1344 wild type S. enterica sv. Typhimurium, SmR [38]
14028s wild type S. enterica sv. Typhimurium [27]
14028s rpoE::cat rpoE deletion, CmR [27]
140282 rpoS::Tn10dCm rpoS deletion, CmR [27]
14028s rpoN::Ap rpoN deletion, AmpR [27]
14028s rpoH::PCN rpoH deletion, temperature sensitive, AmpR [27]
SL1344 ΔrpoE Unmarked, in-frame deletion of rpoE, SmR This work
SL1344 ssrB::3FLAG ssrB-FLAG replacement allele on chromosome This work
SL1344 PsseA::pPsseA-lacZ Merodiploid containing integrated PsseA-lacZ reporter [25]
SL1344 PsseL::pPsseL-lacZ Merodiploid containing integrated PsseL-lacZ reporter This work
SL1344 PsrfN::pPsrfN-lacZ Merodiploid containing integrated PsrfN-lacZ reporter This work
SL1344 PsifA::pPsifA-lacZ Merodiploid containing integrated PsifA-lacZ reporter This work
SL1344 PssaB::pPssaB-lacZ Merodiploid containing integrated PssaB-lacZ reporter This work
SL1344 PssaG::pPssaG-lacZ Merodiploid containing integrated PssaG-lacZ reporter This work
ΔrpoE PsseA::pPsseA-lacZ Merodiploid containing integrated PsseA-lacZ reporter This work
ΔrpoE PsseL::pPsseL-lacZ Merodiploid containing integrated PsseL-lacZ reporter This work
ΔrpoE PsrfN::pPsrfN-lacZ Merodiploid containing integrated PsrfN-lacZ reporter This work
ΔrpoE PsifA::pPsifA-lacZ Merodiploid containing integrated PsifA-lacZ reporter This work
ΔrpoE PssaB::pPssaB-lacZ Merodiploid containing integrated PssaB-lacZ reporter This work
ΔrpoE PssaG::pPssaG-lacZ Merodiploid containing integrated PssaG-lacZ reporter This work
  1. aSm, streptomycin; Amp, ampicillin; Cm, chloramphenicol; Kan, kanamycin