Figure 1

Loss of rpoE changes the abundance of virulence factors in Salmonella. (A) wild type (wt), ΔrpoE, ΔrpoS, and ΔrpoN S. Typhimurium 14028s were grown for 6 hours under SsrB-inducing conditions. Lysates were probed by western blot for SseB, a component of the T3SS needle complex. (B) Western blot was performed as above and lysates probed for SseB in wild type (wt) S. Typhimurium SL1344, ΔrpoE and in ΔrpoE complemented with pWSK29 carrying full length rpoE with endogenous promoters. (C) Wild type S. Typhimurium SL1344 and ΔrpoE cells were immunoblotted as above and lysates probed for SseL-2HA, SrfN-2HA and SifA-2HA which were expressed from their endogenous promoters in pWSK29. Blots were probed for DnaK as a control. The experiment was performed three times with similar results.