Figure 3
From: Quelling targets the rDNA locus and functions in rDNA copy number control
Bidirectional transcription from NTS rDNA locus. Radioactive RT-PCR analysis to detect transcripts derived from NTS rDNA region. Reverse transcription was carried out with specific oligos for NTS rDNA and actin as control and show a signal of the right size from forward and reverse strand of NTS rDNA locus compared to the reaction without reverse transcriptase enzymes. * indicate a signal from genomic rDNA locus (more abundant then actin locus), but that is weak compared to the RT+ lane and therefore reflects the presence of NTS transcripts.