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Table 1 Sequence of primers for PCR, molecular beacon probes and their specific targets

From: Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes

PCR Primers, Probes and Targets Sequence Length Fluorophore/Quencher Tm Probe-target/Stem
RecF 5' GTG GAT CTA TTG TAT TAG ATG AGG CTC TCG 3' 30 - -
RecR 5' GCC AAA GTT CTG CAA CAT TAA CAC CTA AAG 3' 30 - -
NidoF 5' CCA GCC ACA GAA TAC CAT CC 3' 20 - -
NidoR 5' GGA CAT ACT CTG CTG CCA TC 3' 20 - -
Nidogen 5' CGG CGC ACC CAG CTT CGG CTC AGT AGC GCC G 3' 31 TET/BHQ1 77°C/84°C
Nidogen Target 5' ta GGC GCT ACT GAG CCG AAG CTG GGT G at 3' 29 - -
RecA1 5' CCC GCG CGT CTG GCA AGA CTA CTT TAA CTC TTC GCG GG 3' 38 FAM/BHQ1 68°C/71°C
RecA1 Target 5' ta GAA GAG TTA AAG TAG TCT TGC CAG ACG at 3' 31 - -
RecA2 5' CGCGAG TCG TCT GGC AAG ACT ACT TTA A CTCGCG 3' 34 FAM/DABCYL 73°C/67°C
RecA2 Target 5' ttG AGT TAA AGT AGT CTT GCC AGA CGA CTC tt 3' 32 - -
RecA3 5' CTG GCG GAT ATC CTA GGG GG CGC CAG 3' 26 FAM/BHQ1 75°C/75°C
RecA3 Target 5' ttG CGC CCC CTA GGA TAT CCG CCt t 3' 25 - -
  1. Underlined letters in molecular beacons sequence indicate stem sequence and bold letters indicate the probe (loop) sequence. There is an overlaps between probe and stem sequence in RecA3 molecular beacon.
  2. Nucleotides denoted by small case letters in the targets indicate non-template based tails
  3. FAM-Fluorescein, TET-Tetrachlorofluorescein, DABCYL-[4 - ((4 - (dimethylamino)phenyl)azo) benzoic acid] and BHQ-1 = Black Hole Quencher 1