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Figure 3 | BMC Microbiology

Figure 3

From: Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes

Figure 3

SYBR Green 1, a non-specific double stranded nucleotide fluorescent probe, can detect a wide range of B. burgdorferi numbers in the presence of mouse DNA. The amplification plots (A) show PCR of the recA gene of B. burgdorferi strain N40 as detected by SYBR Green at the end of each PCR cycle. Uninfected mouse joint DNA (containing 105 nidogen copies) spiked with a ten-fold dilution of B. burgdorferi DNA, starting with 106 spirochete copies, was used for this assay. A standard curve (B) and a direct correlation (r2 = 0.947) between Ct number and B. burgdorferi number shows that a wide range of spirochete numbers can be detected in our system using SYBR Green.

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