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Table 2 Microarray and real time PCR analysis showing a limited list of genesa predicted to be PreAB activated

From: Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium

ORF Gene Function Microarray Ab Md (fold change) Microarray Bc M (fold change) qRT-PCRe
STM3707 yibD putative glycosyltransferase 0.8 (1.7) 6.1 (68.6) NP f
STM3176 ygiW Membrane protein (DUF388; exporter?) 4.5 (22.6) 5.2 (36.8) 355
STM1253   Cytochrome b561 (Ni2+ dependent) 2.9 (7.5) 4.9 (29.9) 372
STM1595 srfC ssrAB activated gene; predicted coiled-coil structure 4.3 (19.7) 4.7 (26.0) 1.2
STM3175   putative bacterial regulatory helix-turn-helix proteins, AraC family 3.6 (12.1) 4.4 (21.1) 605.3
STM1685 ycjX putative ATPase 2.3 (4.9) 3.8 (13.9) 37.7
STM1252   putative cytoplasmic protein 1.5 (2.8) 2.8 (7.0) 8.6
STM3179 mdaB NADPH specific quinone oxidoreductase (drug modulator) 1.0 (2.0) 2.8 (7.0) 32.5
STM1684 ycjF putative inner membrane protein 1.1 (2.1) 2.6 (6.1) 61.2
STM4291 pmrB sensory kinase in two-component regulatory system with PmrA ND g 2.1 (4.3) NP
STM2080 udg UDP-glucose/GDP-mannose dehydrogenase ND 1.8 (3.5) 23.2
STM4292 pmrA response regulator in two-component regulatory system with PmrB ND 1.7 (3.2) NP
STM4118 yijP (cptA) putative integral membrane protein ND 1.5 (2.8) 32.8
STM0628 pagP PhoP-activated gene, palmitoyl transferase ND 1.1 (2.1) NP
STM2238   putative phage protein 0.9 (1.9) 1.0 (2.0) NP
  1. a This list includes only those genes that were upregulated in both the preA and preAB mutant strains overexpressing preA, those confirmed by real-time PCR, genes previously shown to be preA-regulated (yibD, pmrAB) or those known to belong to the PhoPQ or PmrAB regulons
  2. b ΔpreA/pBAD18-preA vs. ΔpreA/pBAD18
  3. c ΔpreAB/pBAD18-preA vs. ΔpreAB/pBAD18
  4. d M = Log2(expression plasmid/vector control)
  5. e real time PCR (qRT-PCR) performed with cDNA derived from the strains used in Microarray B
  6. f NP = not performed
  7. g ND = not detected