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Table 2 Microarray and real time PCR analysis showing a limited list of genesa predicted to be PreAB activated

From: Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium

ORF

Gene

Function

Microarray Ab

Md (fold change)

Microarray Bc

M (fold change)

qRT-PCRe

STM3707

yibD

putative glycosyltransferase

0.8 (1.7)

6.1 (68.6)

NP f

STM3176

ygiW

Membrane protein (DUF388; exporter?)

4.5 (22.6)

5.2 (36.8)

355

STM1253

 

Cytochrome b561 (Ni2+ dependent)

2.9 (7.5)

4.9 (29.9)

372

STM1595

srfC

ssrAB activated gene; predicted coiled-coil structure

4.3 (19.7)

4.7 (26.0)

1.2

STM3175

 

putative bacterial regulatory helix-turn-helix proteins, AraC family

3.6 (12.1)

4.4 (21.1)

605.3

STM1685

ycjX

putative ATPase

2.3 (4.9)

3.8 (13.9)

37.7

STM1252

 

putative cytoplasmic protein

1.5 (2.8)

2.8 (7.0)

8.6

STM3179

mdaB

NADPH specific quinone oxidoreductase (drug modulator)

1.0 (2.0)

2.8 (7.0)

32.5

STM1684

ycjF

putative inner membrane protein

1.1 (2.1)

2.6 (6.1)

61.2

STM4291

pmrB

sensory kinase in two-component regulatory system with PmrA

ND g

2.1 (4.3)

NP

STM2080

udg

UDP-glucose/GDP-mannose dehydrogenase

ND

1.8 (3.5)

23.2

STM4292

pmrA

response regulator in two-component regulatory system with PmrB

ND

1.7 (3.2)

NP

STM4118

yijP (cptA)

putative integral membrane protein

ND

1.5 (2.8)

32.8

STM0628

pagP

PhoP-activated gene, palmitoyl transferase

ND

1.1 (2.1)

NP

STM2238

 

putative phage protein

0.9 (1.9)

1.0 (2.0)

NP

  1. a This list includes only those genes that were upregulated in both the preA and preAB mutant strains overexpressing preA, those confirmed by real-time PCR, genes previously shown to be preA-regulated (yibD, pmrAB) or those known to belong to the PhoPQ or PmrAB regulons
  2. b ΔpreA/pBAD18-preA vs. ΔpreA/pBAD18
  3. c ΔpreAB/pBAD18-preA vs. ΔpreAB/pBAD18
  4. d M = Log2(expression plasmid/vector control)
  5. e real time PCR (qRT-PCR) performed with cDNA derived from the strains used in Microarray B
  6. f NP = not performed
  7. g ND = not detected