Table 1 primers used in PCR and RT-PCR
From: DNA phosphorothioation in Streptomyces lividans: mutational analysis of the dnd locus
Primer Name | Sequence (with the restriction enzyme sites underlined) | Enzyme site |
|---|---|---|
A2 | ATCACCCCTTCCACCGAGAT | Â |
A1 | ACTGGATGACCGCGGAGTTC | Â |
B1 | GAGTACGTTTTTCCGGCCATCC | Â |
B2 | TCCTTCAGCGCCTGCTCGAT | Â |
B3 | CCAACACCGACTGGGAGGGG | Â |
C1 | CAGAGATCGTCGAGGAGCTG | Â |
C2 | GATCTTCAACCGCTCGGTGC | Â |
C3 | CAGTATCGAACCATGACCCGG | Â |
D1 | TGCGGCAAGACGACCCTGCT | Â |
D2 | GTCGGCGAGCTGTTCCACCT | Â |
D3 | CAGTGATCGACACCCCACTC | Â |
E1 | ATGCCGTCTGAGATCACCAT | Â |
E2 | ATAAGCAGCGTCTTGCCCAC | Â |
16S rRNA SP | AGTAACACGTGGGCAACTGC | Â |
16S rRNA AP | CTCAGACCAGTGTGGCCGGT | Â |
xtg1 | CCGATCTTGTGCCCGCTGATG | Â |
xtg2 | GCGCCTTAAGT CGTCCCTTGTTC | Afl II |
xtg3 | GAAGGTGTCTTAGATCT CCGG | Bgl II |
xtg4 | CTGGCACGACAGGTTTCC | Â |
xtg5 | AAGCACCGGT TCAAGACG | Age I |
xtg6 | GCCCAGGTCCGCAAGAA | Â |
xtg7 | CTCGTGGTTGAGCGGGACTACGG | Â |
xtg8 | CTGGCACCGGT CAAGCCTAGG TG | Age I, Avr II |
xtg9 | GGGACAGCCTAGG GGTGATC | Avr II |
xtg10 | ACTGACCGCAGACCGCAAG | Â |
wlr5 | CATATG GTGGGATCTTCTGCAGCT | Nde I |
wlr6 | GGATCC TCAATGATGATGATGATGATGTGACTCTCCTCGCAGGTA | Bam HI |
wlr7 | CATATG AGCACCCCCAAGGCG | Nde I |
wlr11 | GGATCC TTAGTGGTGGTGGTGGTGGTGTGCAGGTGCATCGGTGGTGA | Bam HI |
dnd-1 | AGAGATCACCACATATG CACCTGAGCACC | Nde I |
dnd-2 | CAGCCGGATCC TGATCTCAG | Bam HI |
dndE-L | CACATATG CCGTCTGAGATCACC | Nde I |
dndE-R | TAAGGCCTATTCGGCGGTGA | Â |