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Table 4 Summary of Igl1 protein levels in Igl shRNA transfectants

From: Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica

shRNA Transfectant or Control Sample % of Igl1 protein level (± SE) P-value
GFP 100.0 ± 3.6  
HM1:IMSS 115.5 ± 11.8 0.1449
Igl (2412–2440) 95.3 ± 3.2 0.2078
Igl1 (272–300) 27.8 ± 1.3 < 0.0001
Igl (1198–1226) 42.3 ± 2.1 < 0.0001
Igl (2777–2805) 38.1 ± 3.1 < 0.0001
  1. The average level of Igl1 protein in the GFP control shRNA transfectants was defined as 100% expression of Igl1 protein for computational purposes. Protein levels of Igl1, using actin as a standard for comparison, were quantified from Western blotting. Values are expressed as the percentage of the GFP level ± SE, with the P-value following each, calculated using Student's t test. For Western blotting, there were three biological replicates, each run in triplicate sets of serial dilutions (1:2, 1:4, and 1:8), with the exception of the HM1:IMSS nontransfected samples having one biological replicate rather than three. Protein levels were not statistically different between the Igl1 (272–300), Igl (1198–1226), and Igl (2777–2805) samples (tested with ANOVA, one-tailed, α = 0.05, 0.1 < P < 0.25) or the GFP, HM1:IMSS, and Igl (2412–2440) samples (tested with ANOVA, one-tailed, α = 0.05, P > 0.25). A representative Western blot is shown in Figure 2.