Western blot for URE3-BP shRNA transfectants. A representative Western blot is shown with three biological replicates each (one dilution shown) for GFP control, URE3-BP (350–378), and URE3-BP (580–608) shRNA transfectants. HM1:IMSS samples are not shown. Results are representative of three biological replicates per shRNA transfectant with each sample run in triplicate. Serial dilutions of the crude lysates (1:2, 1:4, and 1:8) were also done for each sample. Each membrane was probed with anti-actin antibody as a loading control, or with anti-URE3-BP antibody. URE3-BP protein levels are summarized in Table 6.