Table 1 Specific primers used in RT-PCR
From: NF-κB activation by Helicobacter pylorirequires Akt-mediated phosphorylation of p65
Primer | Sequence | Product size (bp) | |
|---|---|---|---|
IL-8 | sense | 5'-ATGACTTCCAAGCTGGCCGTG-3' | 302 |
antisense | 5'-TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC-3' | ||
p65 | sense | 5'-GCGGCCAAGCTTAAGATCTGCCGAGTAAAC-3' | 150 |
antisense | 5'-GCGTGCTCTAGAGAACACAATGGCCACTTGCCG-3' | ||
Akt | sense | 5'-ATGAGCGACGTGGCTATTGTGAAG-3' | 330 |
antisense | 5'-GAGGCCGTCAGCCACAGTCTGGATG-3' | ||
β-actin | sense | 5'-GTGGGGCGCCCCAGGCACCA-3' | 548 |
antisense | 5'-CTCCTTAATGTCACGCACGATTTC-3' |