Skip to main content
Figure 5 | BMC Microbiology

Figure 5

From: NF-κB activation by Helicobacter pylorirequires Akt-mediated phosphorylation of p65

Figure 5

NF-κB-mediated transactivation induced by H. pylori is inhibited by either LY294002 or transfection of a dominant-negative Akt mutant. (A) MKN45 cells were transfected with κB-LUC and phRL-TK for 24 h. Where indicated, the cells were preincubated with LY294002 (20 μM) for 60 min prior to infection with H. pylori (ATCC 49503). They were infected subsequently with H. pylori for 24 h. Luciferase activity was assayed for each sample. Readings were normalized for each sample as expressed κB-LUC over constitutively expressed phRL-TK and plotted as -fold stimulation. (B) Dominant-negative Akt blocked H. pylori signaling to an NF-κB-dependent promoter. MKN45 cells were cotransfected with κB-LUC and phRL-TK, together with either a vector or a construct expressing a dominant-negative Akt (Akt K179A/T308A/S473A). The cells were infected with H. pylori (ATCC 49503) 24 h later. Data are mean ± SD of three independent experiments.

Back to article page