Tc38 patterns in T. cruzi epimastigotes during the cell cycle. Phase contrast, DAPI staining and Tc38 signal are indicated. For the merge images, Tc38-Alexa 488 signal is shown in green and DAPI nucleic acid staining in blue. Selected parasites that show the most frequent patterns seen in the cell cycle phases are presented. A corresponds to G1/S, B to G2 and C to E show images from mitosis to cytokinesis. Each one of the Tc38 labeling patterns were found in the majority of examined cells (n ≥ 20). The arrow indicates the position of the second flagellum, indicative of G2. Black bars = 5 μm. The dotted lines in the phase contrast indicate the position enlarged in the fluorescent images. White bars = 2 μm.