Subcellular localization of Tc38 using immunohistochemical approaches in asynchronous cultures of T. cruzi epimastigotes. DAPI staining and Tc38 signal are indicated. Left panel shows the pooled ISIS software (MetaSystems GmbH) captured image. For the merge image, Tc38-Alexa 488 signal is shown in green and DAPI nucleic acid staining in blue. Bars = 10 μm.