Figure 1

Binding of native Tc38 to different [dT-dG] rich targets. Whole protein extracts of exponentially grown epimastigotes cultures were assayed with oligonucleotide probes representing four putative targets: TG, TEL, MIN and MAX as indicated in Materials and Methods. Reactions were done under the conditions described in Materials and Methods using 1 μg of total epimastigote protein extract, 1 ng (10,000 cpm) of each probe. For each probe, we determined the presence of Tc38 in the complexes by the addition of the anti-Tc38 affinity purified polyclonal antibody (12 μL) to the binding reactions (α-Tc38). A polyclonal antibody against TcPuf6 (12 μL) was used as a control (α-TcPuf6). The presence/absence of the antibodies and protein extract in the binding reactions is indicated by +/- signs above each lane.