Skip to main content

Advertisement

Table 1 Primer sequences, annealing temperatures and product size (RT-PCR).

From: Inducible expression of beta defensins by human respiratory epithelial cells exposed to Aspergillus fumigatusorganisms

Primers Sequences Conditions Product size
hBD1f
hBD1r
5'-agcgtctccccagttcctgaaatcct-3'
5'-tcttctggtcactcccagctcacttg-3'
38 cycles, 61°C 273 bp
hBD2f
hBD2r
5'-catcagccatgagggtcttg-3'
3'-ggctttttgcagcattttgt-3'
38 cycles, 61°C,
2.5% DMSO
199 bp
hBD8f
hBD8r
5'-tactcacctccagccttttgtcatcc-3'
5'-gggtgtagtgctctcaattcttggttg-3'
38 cycles, 61°C 176 bp
hBD9f
hBD9r
5'-tgcagtaagaggtgatttgg-3'
5'-tgacatgataagtggtgttgg-3'
32 cycles, 56°C 174 bp
hBD18f
hBD18r
5'-cctgcttcccaaggaccatgaaactc-3'
5'-ccgagaggaagtcatgagctatggtg-3'
38 cycles, 61°C 400 bp
GAPDHf
GAPDHr
5'-cccatcaccatcttccagagc-3'
5'-ccagtgagcttcccgttcagc-3'
32 cycles, 61°C 473 bp