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Table 1 Primer sequences, annealing temperatures and product size (RT-PCR).

From: Inducible expression of beta defensins by human respiratory epithelial cells exposed to Aspergillus fumigatusorganisms

Primers Sequences Conditions Product size
hBD1f hBD1r 5'-agcgtctccccagttcctgaaatcct-3' 5'-tcttctggtcactcccagctcacttg-3' 38 cycles, 61°C 273 bp
hBD2f hBD2r 5'-catcagccatgagggtcttg-3' 3'-ggctttttgcagcattttgt-3' 38 cycles, 61°C, 2.5% DMSO 199 bp
hBD8f hBD8r 5'-tactcacctccagccttttgtcatcc-3' 5'-gggtgtagtgctctcaattcttggttg-3' 38 cycles, 61°C 176 bp
hBD9f hBD9r 5'-tgcagtaagaggtgatttgg-3' 5'-tgacatgataagtggtgttgg-3' 32 cycles, 56°C 174 bp
hBD18f hBD18r 5'-cctgcttcccaaggaccatgaaactc-3' 5'-ccgagaggaagtcatgagctatggtg-3' 38 cycles, 61°C 400 bp
GAPDHf GAPDHr 5'-cccatcaccatcttccagagc-3' 5'-ccagtgagcttcccgttcagc-3' 32 cycles, 61°C 473 bp