Table 1 Primer sequences, annealing temperatures and product size (RT-PCR).

hBD1f

hBD1r

5'-agcgtctccccagttcctgaaatcct-3'

5'-tcttctggtcactcccagctcacttg-3'

38 cycles, 61°C

273 bp

hBD2f

hBD2r

5'-catcagccatgagggtcttg-3'

3'-ggctttttgcagcattttgt-3'

38 cycles, 61°C,

2.5% DMSO

199 bp

hBD8f

hBD8r

5'-tactcacctccagccttttgtcatcc-3'

5'-gggtgtagtgctctcaattcttggttg-3'

38 cycles, 61°C

176 bp

hBD9f

hBD9r

5'-tgcagtaagaggtgatttgg-3'

5'-tgacatgataagtggtgttgg-3'

32 cycles, 56°C

174 bp

hBD18f

hBD18r

5'-cctgcttcccaaggaccatgaaactc-3'

5'-ccgagaggaagtcatgagctatggtg-3'

38 cycles, 61°C

400 bp

GAPDHf

GAPDHr

5'-cccatcaccatcttccagagc-3'

5'-ccagtgagcttcccgttcagc-3'

32 cycles, 61°C

473 bp