Co-localisation of hBD2 and A. fumigatus organisms. A549 cells were grown on cover slips for 16 h at 37°C. Cells were exposed to RC (A, B, C, D), SC (E, F, G, H) or HF (I, J, K, L) for 18 hours at 37°C. After fixation and permeabilisation, as described for Figure 7, cells were labelled with specific anti-hBD-2 antibody (A, B, E, F, I, J) and secondary antibody conjugated to Texas-red. Normal rabbit serum was used instead of anti-hBD2 as a negative control (C, D, G, H, K, L). Immunofluorescence signal (A, E, I, C, G, K) was compared to phase contrast image of the same cells (B, F, G, D, H, L). Arrows indicated different A. fumigatus morphotypes.