Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli

BMC Microbiology

Table 2 Oligonucleotide primers used in this study

Primer	Sequence (5'-3')
fliC_ΔF	CGTAATCAACGACTTGCAATATAGGATAACGAATCATGGCACAAGTCAT TAATACCTGTGTAGGCTGGAGCTGCTTCG
fliC_ΔR	GCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAAC GACATATGAATATCCTCCTTA
fliC_verifyF	GAGTATTTCGGCGACTAAC
fliC_verifyR	GCAGGGTTTTAATCGGACG
fliI_ΔF	CCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTG GTGTGTAGGCTGGAGCTGCTTCG
fliI_ΔR	GGAGAGACGCTTCCCAGTCCGCGCGTTCAAAAATGCCTTGTTGCAAATAG CCCATATGAATATCCTCCTTA
fliI_verifyF	GCTGGCAAGAACTCTGCCG
fliI_verifyR	GACACTGTCGGGAAAATACGC
fliS_ΔF	GCAGTTCGAAAACAACAGTAATTCCAAGTAATCATTATTCATCGGGAGAC AGGTCTGTGTAGGCTGGAGCTGCTTCG
fliS_ΔR	GCTGGCTTTTTTCGACGAGTTGTTGCCAGGCGAAATATAAATGCGGTGCAT GGTTCACATATGAATATCCTCCTTA
fliS_verifyF	CACGACCACCATCGGCAG
fliS_verifyR	GAATCAGGCGCAGCATCGGGC
fliC F	CATGCCATGGCACAAGTCATTAATACC
fliC R	CGCGGATCCTTAACCCTGCAGCAGAGAC
escF F	GCTCTAGATCTGAGGGAAATTTAATG
escF R	CCCAAGCTTTTAAAAACTACGGTTAGA
espADB_ΔF	GTCATGCTAAGAAAGATTATGAAGAGGTATATACATGGATACATCAACT ACAGCTGTGTAGGCTGGAGCTGCTTCG
espADB_ΔR	GTTCAAGATAGTAATTAAACACTTCATCATTAAACGTATCGACCATGATC AACATATGAATATCCTCCTTA
espADB_verifyF	CCTTCTCGGGTATCGATTGTCG
espADB_verifyR	CGTTAAGCCCTGTTTGGTTACG
pTrc99AF	CGGTTCTGGCAAATATTC

ISSN: 1471-2180