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Table 1 Bacterial strains and plasmids used in this study

From: Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli

Strain/plasmid Characteristic(s)a Motility In DMEM FASb Reference
Strains     
   E2348/69 Wild type EPEC O127:H6 + + [47]
   ICC171 E2348/69 ΔescF (KanR) +/- - [14]
   EPEC ΔespADB E2348/69 ΔespADB (KanR) + - This study
   EPEC ΔfliI E2348/69 Δfli (CmR) - + This study
   EPEC ΔfliC E2348/69 ΔfliC (CmR) - + Grubman et al, submitted
   EPEC ΔfliI/escF E2348/69 ΔfliI/escF (CmR, KanR) - - This study
   EPEC ΔfliI/fliS E2348/69 ΔfliI/fliS (CmR, KanR)    This study
   EPEC ΔsepL E2348/69 ΔsepL (KanR)    [48]
Plasmids     
   pKD46 Red Recombinase helper plasmid (AmpR) N.Ac N.A [45]
   pKD3 Red Recombinase template plasmid (CmR) N.A N.A [45]
   pKD4 Red Recombinase template plasmid (KanR) N.A N.A [45]
   pTrc99A High-copy number expression vector (AmpR) N.A N.A [49]
   pFliC Derivative of pTrc99A encoding fliC from EPEC E2348/69 (AmpR) N.A N.A This study
   pFliCEscF Derivative of pTrc99A encoding fliC and escF from EPEC E2348/69 (AmpR) N.A N.A This study
   pCDNA3 Eukaryotic expression vector N.A N.A Promega
  1. aKan, kanamycin; Cm, chloramphenicol; Amp, ampicillin.
  2. bFAS, Fluorescent actin staining test.
  3. cN.A., not applicable.