Figure 4From: Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coliImmunoblot analysis of secreted proteins (SN) and whole cell lysates (WCL) prepared from derivatives of EPEC E2348/69 grown in hDMEM. Arrows indicate position of a reactive band corresponding to FliC detected with anti-H6 FliC antibodies or DnaK detected with anti-DnaK antibodies. FliC expression was induced in vitro with 1 mM IPTG from the trc promoter in pTrc99A.Back to article page