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Table 1 Composition of biofilms generated under different nutritional conditions.

From: In vitromodeling of host-parasite interactions: the 'subgingival' biofilm challenge of primary human epithelial cells

Culture Conditions

Number of bacteria (± SD) present in biofilm per disc (cfu, ×106)

0-16.5 h

16.5 - 64.5 h

N

Total

Crec

Fnuc

Pgin

Pint

Tfor

Vdis

Anae

Sint

Sora

a

b

15

3450

(1780)

365

(151)

50

(38)

60

(48)

1.4

(1.2)

11

(14)

614

(601)

155

(133)

405

(311)

982

(488)

c

d

12

1770

(719)

171

(140)

36

(61)

2.3

(4.4)

30

(63)

6.8

(7.0)

541

(458)

220

(165)

122

(117)

520

(268)

a

e

12

2710

(1100)

256

(211)

26

(43)

105

(151)

1.7

(3.1)

3.0

(2.7)

931

(702)

103

(122)

219

(190)

686

(309)

a

f

3

710

(529)

1.4

(1.3)

0.01

(0.004)

7.3

(7.6)

0.08

(0.003)

47

(30)

142

(103)

23

(3)

61

(22)

293

(55)

  1. Culture conditions: a, 35% saliva + 35% human serum + 30% FUM with 3% glucose, anaerob (10% H2, 5% CO2, 85% N2); b, 35% saliva + 35% human serum + 30% FUM with 3% glucose, aerob (5% O2, 5% CO2, 90% N2); c, 60% saliva + 10% human serum + 30% FUM with 3% glucose, anaerob (10% H2, 5% CO2, 85% N2); d, 60% saliva + 10% human serum + 30% FUM with 3% glucose, aerob (5% O2, 5% CO2, 90% N2); e, 70% human serum + 30% FUM with 3% glucose, aerob (5% O2, 5% CO2, 90% N2); f, 70% human serum + 30% 0.9% NaCl, aerob (5% O2, 5% CO2, 90% N2). Species abbreviations are as defined in the legend to Figure 1.