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Table 4 Comparison of results for selected up-regulated genes determined by Affymetrix/S score and RQ-PCR.

From: Induction of a chemoattractant transcriptional response by a Campylobacter jejuniboiled cell extract in colonocytes

Gene Description

Ingenuty Name

Affymetrix Probe Set

S Score

Fold RQ-PCR

Network

Location

Interleukin-8

IL8

211506_s_at

11.393

59.4 ± 15.5

See Figure 3

Extra-cellular

ATPase, Na+/K+ transporting, Beta 1 polypeptide

ATP1B1

201242_s_at

7.184

4.5 ± 1.8

10

Plasma Membrane

Syndecan 4

SDC4

202071_at

8.823

4.0 ± 0.84

5

Plasma Membrane

Retinoic acid receptor responder (tazarotene induced) 1

RARRES1

221872_at

6.179

2.4± 0.7

8

Plasma Membrane

tumor necrosis factor, alpha-induced protein 3

TNIP1

207196_s_at

9.344

2.0 ± 0.2

See Figure 3

Nucleus

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NFKBIA

201502_s_at

10.956

4.0 ± 1.2.

See Figure 3

Cytoplasm

Matrix Metallo-peptidase 7

MMP7

202644_s_at

9.812

2.1 ± 4.2

9 & See Additional file 3

Extra-cellular

  1. For each gene ingenuity description, name and Affymetrix probe set, assigned network and cellular location are shown together with the S score and fold RQ-PCR change compared to β-actin control.