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Figure 3 | BMC Microbiology

Figure 3

From: Polymorphism in the flanking regions of the PbGP43 gene from the human pathogen Paracoccidioides brasiliensis: search for protein binding sequences and poly(A) cleavage sites

Figure 3

Radioautograms showing EMSA results with radio labeled (*) Et12, Et23, and Et23Δ probes. When not specified, protein extracts from Pb18 were used. In A, specificity of the EMSA bands was suggested by effective competition with 100 × molar excess of cold homologous probe. In B and D, cross-competition experiments with the indicated cold probes at 100 × or 500 × molar excess. In C, the intensity of Et23 and Et23Δ (mutated in -230 to A) bands are compared with different protein extracts (Pb3 or Pb18, as indicated). In E, migration of Et12 and Et23 bands are compared with protein extracts from different isolates (indicated). The position of shifted bands is indicated with arrows.

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BMC Microbiology

ISSN: 1471-2180

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