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Figure 5 | BMC Microbiology

Figure 5

From: The malate synthase of Paracoccidioides brasiliensisis a linked surface protein that behaves as an anchorless adhesin

Figure 5

Interaction of P. brasiliensis yeast forms with pneumocytes. The interaction was assayed by indirect immunofluorescence and analyzed by flow cytometry. (A) P. brasiliensis yeast cells were pretreated for 1 h with anti-PbMLSr polyclonal antibody (diluted 1:100), and control cells were pretreated with rabbit pre-immune serum. (B) A549 cells were pretreated for 1 h with 25 μg/mL of PbMLSr, and control pneumocytes were pretreated for 1 h with 25 μg/mL of BSA. Adhesion of P. brasiliensis to pneumocytes was analyzed 2 h after the treatments. Infection (adhesion plus internalization) of P. brasiliensis to pneumocytes was analyzed 5 h after the treatments.

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