Figure 3

Localization of Pb MLS by confocal laser scanning microscopy in P. brasiliensis yeast cells growing in different carbon sources. The same groups of cells grown in the presence of potassium acetate (images A and B) or glucose (images C and D) as the sole carbon source are shown, side by side, using differential interference contrast microscopy (DIC) and confocal immunofluorescence. In both situations, the accumulation of PbMLS was restricted to the budding cells. The graph in E displays, comparatively, the immunofluorescence patterns and intensities of two regions of interest (ROI 1 and 2), corresponding to arrows 1 and 2. The data indicate that, under the same labeling conditions, the budding cells cultivated on potassium acetate accumulate PbMLS more intensely on the cell surface than those grown on glucose.