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Figure 2 | BMC Microbiology

Figure 2

From: The malate synthase of Paracoccidioides brasiliensisis a linked surface protein that behaves as an anchorless adhesin

Figure 2

Localization of Pb MLS by confocal laser scanning microscopy in P. brasiliensis yeast cells. Differential accumulation of PbMLS on the surface of budding cells is easily seen in B, C and F. Images A and E represent the differential interference contrast (DIC) of images B and F, respectively. Image C corresponds to a three-dimensional reconstruction of an immunofluorescent tomographic image showing the accumulation of PbMLS only on the budding cells and not in the mother. This is also observed in images B and F. Image G displays the fluorescence pattern and intensity of two regions of interest (ROI) specified by arrows 1 and 2 in image F, indicating that the fluorescence is more intense on the cell surface (2) than in the cytoplasm of budding cells (1). Image D shows a mother cell positive to PbMLS on the cellular surface and the formation, in culture, of budding cells also expressing PbMLS.

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BMC Microbiology

ISSN: 1471-2180

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