Figure 3

Phagocytosis and survival of MS in PKC-α deficient THP-1 cells. THP-1 cells were incubated in the presence of 30 nM PMA for 24 h. Then cells were transfected with 20 nM SiRNA and after 24 h level of PKC were determined by immunoblotting. (A) 24 h after transfection control cells (C) and (ΔA) cells transfected with SiRNA PKCα, (ΔD) cells transfected with SiRNA PKCδ, (S) cells transfected with scrambled SiRNA (PKC-α SiRNA which does not block PKCα), were infected with MS (MOI = 1:10) for 2 h, washed and remaining extracellular bacilli were killed by amikacin treatment for 1 h, again washed, lysed in 0.05% SDS and plated for cfu. 'T' test was performed for statistical analysis of data, (B) 24 h after infection % survival of MS in THP-1 cells transfected with either SiRNA targeting PKC-α (ΔA) or scrambled SiRNA (S), because phagocytosis of MS was different in control and PKC-α deficient cells, cfu at 0 h was considered 100% and survival of MS is presented as percentage of the initial cfu that survive in macrophages after 24 h. (C) 24 h after transfection, level of PKC-δ in cells transfected with SiRNA targeting PKC-δ or scrambled SiRNA, (D) Phagocytosis of MS by mouse macrophage cell line J774A.1 cells pretreated with an inhibitor of PKC-α (Go6976) for 30 minute before infection. Data are means ± standard deviations from three independent experiments each performed in 4 replicates. (*** = p < 0.0001, * = p < 0.05).