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Table 2 Hybridization of well-studied EAEC and DAEC strains to EAEC probes and daaC and results of PCR-RFLP test for daaD and aafB.

From: The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli

Strain

Serotype

Country of isolation/source

HEp-2 adherence pattern*

pAA (CVD 432)

aap

aggA

aafA

daaC

hybrid-ization (SLM 862)

aafB/daaDRFLP

AA 60A

 

Mexico

Aggregative

+

+

+

-

-

-

AA H232-1

 

Peru

Aggregative-detaching

+

+

-

-

-

-

AA 17-2

O3:H2

Chile

Aggregative-detaching

+

+

+

-

-

-

AA 253-1

O3:H2

Thailand

Aggregative

+

+

+

-

-

-

AA 6-1

OR:H2

Thailand

Aggregative

+

+

-

-

-

-

BM369

O86

India

Aggregative

-

-

-

-

-

-

AADS65-R2

 

Philippines

Weak localised-aggregative

-

-

-

-

-

-

AA 501-1

OR:H53

Thailand

Aggregative

-

-

-

-

-

-

AA H223-1

 

Peru

Aggregative

+

+

-

-

-

-

AA DS67-R2

 

Philippines

Aggregative

+

+

+

-

-

-

AA 042

O44:H18

Peru

Aggregative

+

+

-

+

+

aafB

AA 144-1

O77:NM

Thailand

Aggregative-detaching

+

+

-

-

+

aafB

AA 44-1

O36:H18

Thailand

Aggregative

+

+

-

-

-

-

AA H145-1

 

Peru

Aggregative

+

+

+

-

-

-

AA 309-1

O130:H27

Thailand

Aggregative

+

+

+

-

+

aafB

H133

 

Peru

Aggregative

+

-

-

-

-

-

MH46-2

 

Peru

Aggregative

+

+

-

-

-

-

M32-1

 

Peru

Aggregative

-

+

-

-

-

-

C04

 

Nigeria

Aggregative

+

+

-

-

-

-

C08

 

Nigeria

Aggregative

+

+

-

-

-

-

AA 103-1

O148:H28

Thailand

Aggregative

+

+

-

-

-

-

AA 435-1

O33:H16

Thailand

Aggregative

+

+

-

+

+

aafB

AA 199-1

OR:H1

Thailand

Aggregative

+

+

-

+

+

aafB

AA H194-2

 

Peru

Aggregative

+

+

+

-

-

-

AA 278-1

O125ac:H21

Thailand

Aggregative

+

+

-

-

-

-

AA 239-1

OR:H21

Thailand

Aggregative

+

-

-

-

-

-

AA 101-1

O?:H10

Japan

Aggregative

-

-

-

+

+

aafB

G02a

 

Nigeria

Aggregative-detaching

-

-

+

-

-

-

D163

 

UK

Aggregative

-

+

-

-

-

-

AA H92-1

 

Peru

Aggregative

-

-

-

-

-

-

DH5α

 

CVD†

Non-adherent

-

-

-

-

-

-

MG1655

 

CVD†

Non-adherent

-

-

-

-

-

-

DAEC1

 

CVD†

Diffuse

-

-

-

-

+

daaD2

DA57-1186

 

CVD†

Diffuse

-

-

-

-

+

daaD

DA55-2186

 

CVD†

Diffuse

-

-

-

-

+

daaD

DAEC4

 

CVD†

Diffuse

-

-

-

-

+

daaD

TW6350

O157:H45

T. Whittam

Diffuse

-

-

-

-

+

daaD2

DAWC21211

?O81:NM

Thailand

Diffuse

-

-

-

-

-

-

  1. * HEp-2 adherence patterns determined by the 3h assay described by Vial et al. [16]
  2. †CVD = Center for Vaccine Development stocks, University of Maryland, Courtesy of JP Nataro
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BMC Microbiology

ISSN: 1471-2180

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