Table 3 Primers used for genes amplification and sequencing.
Locus | Putative gene Product | Locus position* | Gene size (bp) | Sequence length (bp) | Primers** | Primer sequence 5'-3' |
|---|---|---|---|---|---|---|
aroC | Chorismate synthase | 568275 | 1094 | 433 | 43f | TGGGGCGAAAGCCACGGTCTG |
740r | CCTTCACGGCGTTGATCGACA | |||||
dnaK | Heat shock protein 70 kDa | 818851 | 1910 | 534 | 591f | CACGCTCGCCTGGAAGACGC |
1865r | GGGAACGACCAACTCCTGCGT | |||||
1777r | TCGCTTACGGTCTGGACAAG | |||||
gap | Glyceraldehyde-3- phosphate dehydrogenase | 1259699 | 1007 | 578 | 138f | TCTGCGTTATGACAGCGTTC |
940r | AAGCCCATTCATTGTCGTA | |||||
866r | GAAGACCGAGGAATGGGAGT | |||||
omp25 | 25 kDa outer membrane protein | 2714010 | 641 | 390 | 188f | ACGCGGAACTTGCTTTCGTCG |
649r | GCGCACTCTTAAGTCTCTCG | |||||
recA | Recombinase A | 2079528 | 1085 | 490 | 1f | ATGTCTCAGAATTCATTGCGA |
988r | CTGACGAAGCGTGGTTTCGAT | |||||
697r | ATACGGCGAATATCGAGACG | |||||
rpoB | Beta sub-unit RNA Polymerase | 2046339 | 4133 | 501 | 457f | ATCGTTTCGCAGATGCACCG |
1449r | GACATACGTTCCTTGATCGCG | |||||
1119r | TGACGCGATAGATGTCGAAC | |||||
trpE | Anthranilate synthase | 1671911 | 2195 | 564 | 15f | TGCGGATAGCGAGATATTCCA |
1486r | GCCGATGCCTTCAATTCGGT | |||||
659r | GTTGCCGTGCGAGACCAT |