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Table 3 Oligonucleotide primers used in this study

From: YsxC, an essential protein in Staphylococcus aureus crucial for ribosome assembly/stability

Primer

Sequence (5' → 3')

5'GLUSh3I

ataaGGATCCtggcctgtttaataggatct1

3'GLUSh3I

ataaGGATCCaacttgtagcaggaagtggt1

3'GLUSh6A

taaatAAGCTTaattgtgagcggctcacaattccac1

5'GLUSh6A1

tattaaGCGGCCGCtcattgcttccaaggagctaaagaggtccctag1

3'GLUSh6B

atattAAGCTTagaaatccctttgagaatgttt1

5'GLUSh6B1

tattaaGCGGCCGCcggattttatgaccgatgatgaag1

5'GLUSh16H

attaattcaatattattaggattaactttcattttatatcctcactt aattgtgagcggctcacaattccac2

3'GLUSh16H

ttcaaatattatataatggtagagttgaaagagaatataaaa ttagaaatccctttgagaatgtt2

5'GLUSh65B

cttacattatttttaaaatttttgtataagttttgtcgtacaaaaa atcgatacaaattcctcg2

3'GLUSh65B

ataataaacaacaacaaatatggaatttaattgaaccgtatatttca atggaaaagagaagatgg2

5'GLUSh27A

aattgGGCGCGCCatggaaaagagaagatgg1

3'GLUSh27A

atttGCGGCCGCtcaggttgacttccccgcgg1

5'GLUSh27B

atttGCGGCCGCgataaacccagcgaaccattg1

3'GLUSh27B

atttGGCCGGCCatcgatacaaattcctcg1

5'GLUSh103A

taatgtATCGATaataatggtttcttagacg1

3'GLUSh103A

tattatGTCGACagtcggcattatctc1

5'elc4

atgaaagttaatcctaataatattg3

3'elc4

ttacaccaccaccaccaccactgaaatatacggttcaattaaattc3

  1. 1 upper case bases indicate restriction sites engineered within the oligonucleotide
  2. 2 italics indicate the fragment of the oligonucleotide designed for λred recombination, whilst non-italics indicate the portion of the primer designed to amplify the insert; blackboxes indicate the location of the RBS and the START of ysxC in the complementary strand (5'GLUSh16H) or the 3' end of the ysxC sequence (3'GLUSh65B).
  3. 3 for 3'-dA overhang ligation