Figure 1
From: YsxC, an essential protein in Staphylococcus aureus crucial for ribosome assembly/stability
Detailed scale representation of the P spac ~ ysxC (LC108/LC109) and ysxC ::TAP-tag (LC103) chromosomal constructs. λred recombination allowed highly specific chimera construction resulting in the Tet-T-Pspac or TAP-tag-kan cassette insertions. The relevant sequence junctions are shown for both constructs. Chromosomal sequence is shown in italics and relevant features generated by λred recombination are underlined.